Composite

Part:BBa_K4130000:Design

Designed by: Sarah Broas   Group: iGEM22_Rochester   (2022-09-28)


IgG F(c) Binding Protein, EibD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1482
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed a genetic circuit composed of the EibD gene from E. coli strain ECOR-9, a rhamnose-inducible promoter (BBa_K914003), strong ribosome binding site (BBa_B0034), and double-terminator (BBa_B0015). Synthesized parts were assembled into the chloramphenicol-resistance conferring pSB1C3 vector.


Source

E. coli strain ECOR-9, genomic

References